2025

  1. Fung HYJ, Jiou J, Niesman AB, Bernardes NE, Chook YM. Nap1 and Kap114 co-chaperone H2A-H2B and facilitate targeted histone release in the nucleus. J Cell Biol. 2025 Jan 6;224(1):e202408193.

    *Video Summary*

2024

  1. Hudman-Wing CE, Fung HYJ, Cagatay T, Kwanten B, Niesman AB, Gharghabi M, Permentier B, Shakya B, Shacham S, Landesman Y, Lapalombella R, Daelemans D, Chook YM. Allosteric degraders induce CRL5 ASB8 mediated degradation of XPO1. bioRxiv [Preprint]. 2024 Oct 11:2024.10.07.617049.
  2. Fung HYJ, Mittal SR, Niesman AB, Jiou J, Shakya B, Yoshizawa T, Cansizoglu AE, Rout MP, Chook YM. Phosphate-dependent nuclear export via a novel NES class recognized by exportin Msn5. bioRxiv [Preprint]. 2024 Aug 12:2024.08.12.607649.
  3. Bernardes NE, Chook YM. WW domains: A globular NLS recognized by importin-α. J Cell Biol. 2024 Jun 3;223(6):e202405003. doi: 10.1083/jcb.202405003. Epub 2024 May 20.

2023

  1. Gonzalez A, Kim HJ, Freibaum BD, Fung HYJ, Brautigam CA, Taylor JP, Chook YM. A new Karyopherin-β2 binding PY-NLS epitope of HNRNPH2 linked to neurodevelopmental disorders. Structure. 2023 Aug 3;31(8):924-934.e4.
  2. Shaffer JM, Jiou J, Tripathi K, Olaluwoye OS, Fung HYJ, Chook YM, D'Arcy S. Molecular basis of RanGTP-activated release of Histones H2A-H2B from Importin-9. Structure. 2023 Aug 3;31(8):903-911.e3.
  3. Jiou J, Shaffer JM, Bernades NE, Fung HYJ, Kikumoto Dias J, D'Arcy S, Chook YM. Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complex. Proc Natl Acad Sci U S A. 2023 Jul 18;120(29):e2301199120.
  4. Korff A, Yang X, O'Donovan K, Gonzalez A, Teubner BJ, Nakamura H, Messing J, Yang F, Carisey AF, Wang YD, Patni T, Sheppard H, Zakharenko SS, Chook YM, Taylor JP, Kim HJ. A murine model of hnRNPH2-related neurodevelopmental disorder reveals a mechanism for genetic compensation by Hnrnph1. J Clin Invest. 2023 Jul 17;133(14):e160309.

2022

  1. Bernardes NE, Fung HYJ, Li Y, Chen Z, Chook YM. Structure of IMPORTIN-4 bound to the H3-H4-ASF1 histone-histone chaperone complex. Proc Natl Acad Sci U S A. 2022 Sep 20;119(38).
  2. Fung HYJ, Chook YM. Crystallization of Nuclear Export Signals or Small-Molecule Inhibitors Bound to Nuclear Exporter CRM1. Methods Mol Biol. 2022;2502:285-297.
  3. Fung HYJ, Chook YM. Binding Affinity Measurement of Nuclear Export Signal Peptides to Their Exporter CRM1. Methods Mol Biol. 2022;2502:245-256.
  4. Wing CE, Fung HYJ, Chook YM. Karyopherin-mediated nucleocytoplasmic transport. Nat Rev Mol Cell Biol. 2022 Jan 20.

2021

  1. Fung HYJ, Niesman A, Chook YM. An update to the CRM1 cargo/NES database NESdb. Mol Biol Cell. 2021 Mar 15;32(6):467-469.
  2. Gonzalez A, Mannen T, Çağatay T, Fujiwara A, Matsumura H, Niesman AB, Brautigam CA, Chook YM, Yoshizawa T. Mechanism of karyopherin-β2 binding and nuclear import of ALS variants FUS(P525L) and FUS(R495X). Sci Rep. 2021 Feb 12;11(1):3754.

    3. *Sketch*

  3. Walker JS, Hing ZA, Harrington B, Baumhardt J, Ozer HG, Lehman A, Giacopelli B, Beaver L, Williams K, Skinner JN, Cempre CB, Sun Q, Shacham S, Stromberg BR, Summers MK, Abruzzo LV, Rassenti L, Kipps TJ, Parikh S, Kay NE, Rogers KA, Woyach JA, Coppola V, Chook YM, Oakes C, Byrd JC, Lapalombella R. Recurrent XPO1 mutations alter pathogenesis of chronic lymphocytic leukemia.J Hematol Oncol. 2021 Jan 15;14(1):17.

2020

  1. Natalia Elisa Bernardes, Yuh Min Chook; Nuclear import of histones. Biochem Soc Trans 18 December 2020; 48 (6): 2753–2767.
  2. Baumhardt JM, Walker JS, Lee Y, Shakya B, Brautigam CA, Lapalombella R, Grishin N, Chook YM. Recognition of nuclear export signals by CRM1 carrying the oncogenic E571K mutation. Mol Biol Cell. 2020 Aug 1;31(17):1879-1891.

    3. *Science Sketch*

  3. Springhower CE, Rosen MK, Chook YM. Karyopherins and condensates. Curr Opin Cell Biol. 2020 Jun;64:112-123. doi: 10.1016/j.ceb.2020.04.003. Epub 2020 May 28.
  4. Lee Y, Baumhardt JM, Pei J, Chook YM, Grishin NV. pCRM1exportome: database of predicted CRM1-dependent Nuclear Export Signal (NES) motifs in cancer-related genes. Bioinformatics. 2020 Feb 1;36(3):961-963.

2019

  1. Lee Y, Pei J, Baumhardt JM, Chook YM, Grishin NV. Structural prerequisites for CRM1-dependent nuclear export signaling peptides: accessibility, adapting conformation, and the stability at the binding sites. Sci Rep. 2019 Apr 29;9(1):6627.
  2. Padavannil A, Sarkar P, Kim SJ, Cagatay T, Jiou J, Brautigam CA, Tomchick DR, Sali A, D'Arcy S, Chook YM. Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone. Elife. 2019 Mar 11;8.

2018

  1. Zhang K., Shang G., Padavannil A., Wang J., Sakthivel R., Chen X., Kim M., Thompson M.G., García-Sastre A., Lynch K.W., Chen Z.J., Chook Y.M. and Fontoura B.M.A. Structural-functional interactions of NS1-BP protein with the splicing and mRNA export machineries for viral and host gene expression. (2018) Proc Natl Acad Sci U S A, 115(52):E12218-E12227.
  2. Fu S.C., Fung H.Y.J., Çağatay T., Baumhardt J. and Chook Y.M. Correlation of CRM1-NES affinity with nuclear export activity. (2018) Mol Biol Cell, 29(17):2037-2044.
  3. Çağatay T. and Chook Y.M. Karyopherins in cancer. (2018) Curr Opin Cell Biol, 52:30-42.
  4. Pinarbasi E.S., Cağatay T., Fung H.Y.J., Li Y.C., Chook Y.M. and Thomas P.J. Active nuclear import and passive nuclear export are the primary determinants of TDP-43 localization. (2018) Sci Rep, 8(1):7083.
  5. Yoshizawa T., Ali R., Jiou J., Fung H.Y.J., Burke K.A., Kim S.J., Lin Y., Peeples W.B., Saltzberg D., Soniat M., Baumhardt J.M., Oldenbourg R., Sali A., Fawzi N.L., Rosen M.K. and Chook Y.M. Nuclear Import Receptor Inhibits Phase Separation of FUS through Binding to Multiple Sites. (2018) Cell, 173(3):693-705.
  6. Guo L., Kim H.J., Wang H., Monaghan J., Freyermuth F., Sung J.C., O'Donovan K., Fare C.M., Diaz Z., Singh N., Zhang Z.C., Coughlin M., Sweeny E.A., DeSantis M.E., Jackrel M.E., Rodell C.B., Burdick J.A., King O.D., Gitler A.D., Lagier-Tourenne C., Pandey U.B., Chook Y.M., Taylor J.P. and Shorter J. Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains. (2018) Cell, 173(3):677-692.

2017

  1. Fung, H.Y., Fu, S.C. and Chook, Y.M. Nuclear export receptor CRM1 recognizes diverse conformations in nuclear export signals. (2017) eLife, 6:e23961.

2016

  1. Hing, Z.A., Fung, H.Y., Ranganathan, P., Mitchell, S., El-Gamal, D., Woyach, J.A., Williams, K., Goettl, V.M., Smith, J., Yu, X., Meng, X., Sun, Q., Cağatay, T., Lehman, A.M., Lucas, D.M., Baloglu, E., Shacham, S., Kauffman, M.G., Byrd, J.C., Chook, Y.M., Garzon, R. and Lapalombella, R. Next-generation XPO1 inhibitor shows improved efficacy and in vivo tolerability in hematological malignancies. (2016) Leukemia, 30(12):2364-2372.
  2. Soniat, M. and Chook, Y.M. Karyopherin-β2 Recognition of a PY-NLS Variant that Lacks the Proline-Tyrosine Motif. (2016) Structure, 24(10):1802-1809.
  3. Soniat, M., Çağatay T. and Chook, Y.M. Recognition Elements in the Histone H3 and H4 Tails for Seven Different Importins. (2016) J Biol Chem, 291(40):21171-21183.
  4. Volpon, L., Culjkovic-Kraljacic, B., Osborne, M.J., Ramteke, A., Sun, Q., Niesman, A., Chook, Y.M. and Borden K.L. Importin 8 mediates m7G cap-sensitive nuclear import of the eukaryotic translation initiation factor eIF4E.(2016) Proc Natl Acad Sci U S A, 113(19):5263-8.

2015

  1. Fung, H.Y., Fu, S.C., Brautigam, C.A. and Chook, Y.M. Structural determinants of nuclear export signal orientation in binding to exportin CRM1. (2015) eLife;4:e10034.
  2. Soniat, M. and Chook, Y.M. Nuclear localization signals for four distinct karyopherin-β nuclear import systems. (2015) Biochemical Journal, 468(3):353-362.
  3. Xu, D., Marquis, K., Pei, J., Fu, S.C., Cağatay, T., Grishin, N.V. and Chook, Y.M. LocNES: a computational tool for locating classical NESs in CRM1 cargo proteins.(2015) Bioinformatics, 31(9):1357-1367.
  4. Haines, J.D., Herbin, O., Moy, G.A., Vidaurre, O.G., Adula, K., Sun, Q., Fung, H.Y.J., Albrecht, S., Alexandropoulos, K., McCauley, D., Chook, Y.M., Kuhlmann, T., Kidd, G.J., Shacham, S. and Casaccia, P. Nuclear export inhibitors avert progression in preclinical models of inflammatory demyelination. (2015) Nat. Neuroscience, 18(4):511-520.

2014

  1. Fung, H. Y. J. and Chook, Y. M. Atomic basis of CRM1-cargo recognition, release and inhibition. (2014) Seminars in Cancer Biology, 27:52-61.
  2. Bernis, C., Swift-Taylor, B., Nord, M., Carmona, S., Chook, Y.M., Forbes, D.J. Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration. (2014) Mol Biol Cell. 25(7):992-1009.

2013

  1. Soniat, M., Sampathkumar, P., Collett, G., Gizzi, A.S., Banu, R.N., Bhosle, R.C., Chamala, S., Chowdhury, S., Fiser, A., Glenn, A.S., Hammonds J., Hillerich, B., Khafizov, K., Love, J.D., Matikainen, B., Seidel, R.D., Toro, R., Rajesh Kumar, P., Bonanno J.B., Chook, Y.M., Almo, S.C. Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2. (2013) J Struct Funct Genomics, 14(2):31-5.
  2. Sun, Q, Carrasco, Y.P., Hu, Y., Guo, X., Mirzaei, H., Macmillan, J., Chook, Y.M. Nuclear export inhibition through covalent conjugation and hydrolysis of Leptomycin B by CRM1. (2013) Proc. Natl. Acad. Sci. USA, 10(4):1303-8.
  3. Etchin, J. Sun, Q., Kentsis A., Farmer A., Zhang Z.C., Sanda T., Mansour M.R., Barcelo C., McCauley D., Kauffman M., Shacham S., Christie A.L., Kung A.L., Rodig S.J., Chook Y.M., and Look A.T. Antileukemic activity of nuclear export inhibitors that spare normal hematopoietic cells. (2013) Leukemia, 27(1):66-74.

2012

  1. Lapalombella, R., Sun, Q., Williams, K., Tangeman, L., Jha, S., Zhong, Y., Goettl, V., Mahoney, E., Berglund, C., Gupta, S., Farmer, A., Mani, R., Johnson, A.J., Lucas, D., Mo, X., Daelemans, D., Sandanayaka, V., Shechter, S., McCauley, D., Shacham, S., Kauffman, M., Chook, Y.M., Byrd, J.C. Selective inhibitors of nuclear export show that CRM1/XPO1 is a target in chronic lymphocytic leukemia. (2012) Blood, 120(23):4621-34.
  2. Xu, D., Grishin N.V. and Chook, Y.M. NESdb: a database of NES-containing CRM1 cargoes. (2012) Mol. Biol. Cell, 23(18):3673-6.
  3. Xu, D., Farmer, A. , Collet G., Grishin N.V. and Chook, Y.M. Sequence and structural analyses of nuclear export signals in the NESdb database. (2012) Mol. Biol. Cell, 23(18):3677-93.
  4. Zhang Z.C. and Chook Y.M. Structural and energetic basis of ALS-causing mutations in the atypical proline-tyrosine nuclear localization signal of the Fused in Sarcoma protein (FUS). (2012) Proc. Natl. Acad. Sci. USA, 109(30):12017-21

2011

  1. Zhang Z.C., Satterly N., Fontoura B.M., and Chook Y.M. Evolutionary development of redundant nuclear localization signals in the mRNA export factor NXF1. (2011) Mol. Biol. Cell, 22(23):4657-68.
  2. Chook, Y. M. and Süel, K. E. Nuclear import by karyopherin-βs: recognition and inhibition. (2011) Biochim Biophys Acta. 1813(9):1593-606.
  3. Ge Q, Nakagawa T, Wynn RM, Chook YM, Miller BC, Uyeda K. Importin-alpha protein binding to a nuclear localization signal of carbohydrate response element-binding protein (ChREBP). J Biol Chem. 2011 Aug 12;286(32):28119-27.

2010

  1. Xu, D., Farmer, A. and Chook, Y.M. Recognition of nuclear targeting signals by Karyopherin-β proteins. (2010) Curr Opin Struct Biol. 20(6):782-90.

2009

  1. Süel, K. E. and Chook, Y. M. Kap104p imports the PY-NLS-containing transcription factor Tfg2p into the nucleus. (2009) J. Biol. Chem. 284(23):15416-24.
  2. Dong, X., Biswas, A. and Chook, Y. M. (2009) Structural basis for assembly and disassembly of the CRM1 nuclear export complex. Nat. Struc. & Mol. Biol., 16(5):558-560.
  3. Dong, X., Biswas, A., Süel, K. E, Jackson, L. K., Martinez, R., Gu, H. and Chook, Y. M. Structural basis for leucine-rich nuclear export signal recognition by CRM1. (2009) Nature, 458: 1136-1141.

2008

  1. Zhang, Z. C. and Chook, Y. M. Structural Analysis of Karyopherin-mediated nucleocytoplasmic transport. (2008) Nuclear Transport, edited by Ralph Kehlenbach, Landes Bioscience.
  2. Süel, K. E., Gu, H. and Chook, Y. M. Modular organization and combinatorial energetics of proline-tyrosine nuclear localization signals. (2008) PLoS Biology, 6(6):e137.

2007

  1. Cansizoglu, A. E. and Chook, Y. M. Conformational heterogeneity of Karyopherinβ2 is segmental. (2007) Structure, 15(11):1431-1441.
  2. Cansizoglu, A. E., Lee, B. J., Zhang, Z. C., Fontoura, B. M. A. and Chook, Y. M. Structure-based design of a pathway-specific nuclear import inhibitor. (2007) Nat. Struc. & Mol. Biol., 14(5):452-454.

2006

  1. Lee, B. J., Cansizoglu, A. E., Süel, K. E., Louis, T. H., Zhang, Z. and Chook, Y. M. Rules for nuclear localization sequence recognition by Karyopherinβ2. (2006) Cell, 126:543-558.
  2. Süel, K. E., Cansizoglu, A. E. and Chook, Y. M. Atomic resolution structures in nuclear transport. (2006) Methods, 39(4):342-5.
  3. Faria, A.M.C, Levay, A., Kamphorst, A. O., Rosa, M. L. P., Nussenzveig, D. R., Balkan, W., Chook, Y. M., Wang, Y., Levy, D. E., and Fontoura, B.M.A. The nucleoporin Nup96 is required for proper expression of interferon-regulated proteins and functions. (2006) Immunity, 24(3):295-304.

2004

  1. Wang W., Yang X., Kawai T., de Silanes I.L., Mazan-Mamczarz K., Chen P , Chook Y.M., Quensel C., Kohler M. and Gorospe M. AMP-activated protein kinase-regulated phosphorylation and acetylation of importin alpha1: involvement in the nuclear import of RNA-binding protein HuR. (2004) J. Biol Chem. 2004 279(46):48376-88.

2002

  1. Chook, Y.M., Jung, A., Rosen, M.K. and Blobel, G. Uncoupling Ran binding and substrate dissociation in the Karyopherin b2 nuclear import pathway. (2002) Biochemistry 41, 6955-6966.

2001

  1. Chook, Y.M. and Blobel, G. Karyopherins and nuclear import. (2001) Current Opinions in Structural Biology 11(6):703-715.

1999

  1. Chook, Y.M., Cingolani, G., Conti, E., Stewart, M., Vetter, I., Wittinghofer, A. Pictures in cell biology. Structures of nuclear-transport components. (1999) Trends Cell Biol. 9(8), 310-311.
  2. Chook, Y.M. and Blobel, G. Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp. (1999) Nature 399, 230-237.

1998

  1. Chook, Y.M., Lipscomb, W.N. and Ke, H. Detection and Use of Pseudo Translation in Determination of Protein Structures. (1998) Acta Cryst. D54, 822-827.

1997

  1. Lipscomb, W.N., Chook, Y.M. and Ke, H. Chorismate Mutase, essentially a template enzyme. (1997) Chapter 17, 235-244. Biological NMR Spectroscopy. John L. Markley, Stanley Opella (editors) and Richard Ernst. Oxford University Press.
  2. Kubiseski, T.J., Chook, Y.M., Parris, W.E., Rozakis-Adcock, M. and Pawson, T. High affinity binding of the pleckstrin homology domain of mSos1 to phosphatidylinositol (4,5)-bisphosphate. (1997) J. Biol. Chem. 272 (3), 1799-1804.

1996

  1. Chook, Y.M., Gish, G.D., Kay, C.Y., Pai, E.F. and Pawson, T. The Grb2-mSos1 Complex Binds Phosphopeptides with Higher Affinity than Grb2. (1996) J. Biol. Chem. 271(48), 30472-30478.

1994

  1. Chook, Y.M., Ke, H., Gray, J.V. and Lipscomb, W.N. The monofunctional chorismate mutase from Bacillus subtilis: Structure determination of chorismate mutase and its complexes with a transition state analog and prephenate, and implications on the mechanism of enzymatic reaction. (1994) J. Mol. Biol. 240(5), 476-500.

1993

  1. Chook, Y.M., Ke, H. and Lipscomb, W.N. Crystal structures of the monofunctional chorismate mutase from Bacillus subtilis and its complex with a transition state analog. (1993) Proc. Natl. Acad. Sci. USA, 90, 8600-8603.

1991

  1. Stevens, R.C., Chook, Y.M., Cho, C.Y., Lipscomb, W.N. and Kantrowitz, E.R. Escherichia coli aspartate carbamoyltransferase: The probing of crystal structure analysis via site-specific mutagenesis. (1991) Protein Engineering 4(4), 391-408.

1990

  1. Chey, J., Choe, H.S., Chook, Y.M., Jensen, E., Seida, P.R. and Francl, M.M. π-Complexes of alkenes to trivalent aluminum. (1990) Organometallics, 9(9), 2430-2436.